Composite

Part:BBa_M36304:Design

Designed by: Darren Jindal, Raman Nelakanti, Dennis Te   Group: Stanford BIOE44 - S11   (2011-12-08)

Constitutive promoter with FseI restriction sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 20
    Illegal NgoMIV site found at 93
    Illegal NgoMIV site found at 109
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The strong promoter was surrounded by four cut sites to increase the chance of FseI cutting the promoter on either side.

Source

References